Diagnosis of HCV infection in renal chronic infection patients by using ELSA and RT-PCR in Tikrit City

Viral hepatitis infection is an important cause of mortality and morbidity among patients treated hemodialysis and the spread of this disease varies from one region to another in the world.cross-sectional prospective study conducted in liver and digestive system Hospital in the city of Baghdad.

The study was conducted in the laboratories of the liver and digestive system Hospital and in the city of Baghdad for the period from 1/1/2017 to 1/1/2018, which included the diagnosis of infection with viral hepatitis C of serum patients using the technique of Elisa and RT-PCR for patients who are deceased and incoming to Tikrit Hospital and clinics In the study, 100 samples were taken from 50 patients with renal failure and 50 healthy people aged 50–60 years, with clinical signs and according to the doctor's diagnosis, 5 ml of blood and serum were withdrawn into two parts (1 ml) For the purpose of isolating DNA and detecting the virus with RT-PCR technology and the second to purpose virus detection and enzyme Liver and kidney function using ELISA technique.

The study result appearance Of the 50 people with chronic renal failure, 35(70%) had positive an anti-HCV, while 15 (30%) had negative an anti-HCV antigen. While the results of renal function and liver enzymes showed a significant difference at P≥ 0.05 level and between infection with the C virus except for the GPT enzyme did not show a significant difference at the level P≥ 0.05, and the results also showed relationship between age and infection with the virus, which affects most age groups between 40–50 Years. HCV-RNA levels were also determined in positive and negative serum samples for ELISA testing using RT-PCR the total positive individuals 2.9% (n=1) patients were positive for HCV by RT-PCR and negative of ELISA, while 28.6% (n = 10) were positive cases of HCV by ELISA but not by PCR.