The studies on callus initiation and plantlet regeneration in field bean were undertaken by using B5 medium, ten genotypes and different growth regulators. The stem and leaf explants were used for callus induction, while shoot-tip and cotyledonary node explants were used for plantlet regeneration. The response for per cent callus induction and plantlet regeneration varied with genotype, explants and growth regulators. It was observed that the genotype Konkan Wal 1, explant stem and growth regulator combination of 2.5 mg/l IAA + 5.0 mg/I BA recorded maximum callus induction. The genotype Konkan Wal 1, explant shoot-tip and growth regulator level of 0.1 mg/l BA recorded maximum plantlet regeneration.
Callus, field bean, growth regulator, regeneration
