Department of Vegetable Science, G.B. Pant University of Agriculture and Technology, Pantnagar-263145
*Corresponding author's present address: Training Associate (Horticulture), Forage Unit, RVC, Birsa Agri. University, Konke,Ranchi-834007, Jharkhand
Tubers of two developmental stages, viz. 45 days after planting (DAP) (>5 g) and 80 DAP (25-30 g) were collected from five Indian genotypesviz., JX-1, MF-1, 85-P-ll, 85-P-670 and 85-P-718 grown in field conditions during Rabi 2000-2001. The protein was extracted and electrophoresed through 12.5% acrylamide SDS gels. Results indicated that protein composition in tubers 5g at 45 DAP of two stages was quite different in 40-68 kD and 20-35 kD regions. Banding patterns in small developing tubers >5g at 45 DAP were almost uniform across the genotypes. Absence of polypeptides in 20-35 kD and presence of dark bands in 35-40 kD and below 14 kD regions in mature tubers of 25-30g weight at 80 DAP were reproducible and could be used for variability studies. Heat treatment to the crushed tubers before centrifugation resulted in destruction of protein subunits.
Potato, protein subunits, Solanum tuberosum
