Biochemical parameters of seeds of parents and hybrid of cotton

The seed protein fractions, their profiles through electrophoresis, protease activity and enzyme proteins were examined in hybrid and its parents in cotton. The amounts of protein fractions were intermediate in hybrid to its parent except prolamin and total proteins which showed maximum and minimum values respectively. The total proteins in the seeds were maximum and prolamins were minimum. For protein profiles, no band was observed in the prolamin-like substance fraction. In other fractions, bands differed in their intensities. Qualitative analysis through electrophoresis in terms of presence and absence and differences in the intensities of the bands in different profiles of storage proteins proved to be an important parameter to differentiate parents and hybrid of cotton.