In Vitro Shoot multiplication of tecomella undulata (SM.) SEEM. - AN Endangered Tree Species

An efficient protocol for in vitro propagation of Tecomella undulata through multiple shoot formation from nodal segment explants has been developed. MS basal medium+1.5 mg l⁻ BAP+0.02 mg l⁻¹ IAA was found to be the most effective media for maximum (95%) regeneration of nodal explant at 25±1°C and 16/8 hours cycle of light (2000 lux fluorescent tubes) and dark. Nodal segment showed maximum (9) shoot induction per explant. Twenty-nine shoots per nodal segment explant were observed on MS+0.75 mg l⁻¹ BAP+0.01 mg l⁻¹ IAA within 3 weeks. The rooting percentage (66%) and average number of days (8) for root induction were recorded by following the two-step procedure. In the first step, a 48 hour treatment of ½ MS medium (liquid) + 2.5 mg l⁻¹ IBA was given to isolated shoots. In the second step these shoots were transferred to hormone free ½ MS medium. Seventy-three per cent survibility of plantlets was recorded in potting mixture composed of drained soil+vermiculite (3: 1, v/v).