Production of Diphenol and Monophenol compounds from the leaf callus of Morus Alba and studies on their antibacterial properties
Abstract
White mulberry (Morus alba L.) is an economically important tree species of family Moraceae and is cultivated in many parts of the world. Leaf discs of white mulberry were used as explants and inoculated on to Murashige and Skoog (MS) medium supplemented with various concentrations of 2,4-D, NAA, BAP and KiN. Antibacterial activity was evaluated from explants and leaf induced calluses against Bacillus subtilis. Calluses raised from different combinations showed remarkable antibacterial activity against Bacillus subtilis as compared to explants alone i.e. leaf tissue. Phenolic contents from explants and calluses were also estimated. Diphenols and monophenols were higher in callus cells as compared to explants suggesting their probable role as antimicrobial agents against B. subtilis. Further, antibacterial activity against B. subtilis was also tested with standard monophenol and diphenol.
Keywords
Antibacterial activity, Bacillus subtilis, leaf callus, Morus alba, phenols
