Natural Products Department, Regional Research Laboratory, Bhubaneswar - 751013
*Corresponding author
Nodal stem segments from one year old plants of Desmodium gangeticum were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of BA and IAA. Explants cultured on MS basal medium supplemented with 1.0 mg/l BA + 0.25 mg/l IAA showed highest rate of shoot multiplication. In vitro shootlets were rooted on to the half-strength MS basal media supplemented with 1.0 mg/l IBA or IAA. Rooting was better in IBA supplemented medium than IAA. Rooted shoots were transplanted in the greenhouse for hardening and their survival was 80 % in the field condition.
Key words: Desmodium gangeticum, medicinal plant, micropropagation, nodal segment
