Micropropagation of Acmella Calva (DC) R. K. Jansen from node explants

Efficient method for plant regeneration from nodal explants of Acmella calva (DC) R. K. Jansen has been developed. The nodal explants were cultured on Murashige and Skoog’s (MS) medium fortified with different concentrations (0.5 – 5.0 mg l⁻¹) of benzyldenine (BA), and adenine sulphate (Ads). BA was found to be the effective cytokinin, which when used at 3.0 mg l⁻¹, produced 15 shoots/explant. The well developed shoots were excised and rooted on ½ and full strength MS medium containing different concentrations (0.5 – 5.0 mg l⁻¹) of IAA and NAA. The rooted plantlets were transferred to plastic cups containing vermiculite for hardening and then to the soil condition. After one month the in vitro derived plantlets started to produce flowers.