Biochemical changes during shoot differentiation in callus cultures of Chlorophytum borivilianum sant. et fernand

Biochemical changes leading to shoot regeneration during in vitro culture of callus derived from bud pedicel of Chlorophytum borivilianum on MS medium (Murashige and Skoog, 1962) supplemented with 1.0 mg/l BAP + 1.0 mg/l NAA were investigated. Starch content and reducing sugars were high in the control callus, which further increased significantly in shoot differentiating cultures. Contents of total soluble sugars, free amino acids, total soluble proteins and total phenols were lower in the former and increased in the shoot differentiating cultures. The activities of enzymes like-amylase, acid-protease, acid-phosphatase and peroxidase increased up to appearance of green patches (8–12 day) and reached a peak on 16^th day of inoculation that coincided with the appearance of shoots. Conversely, the acid invertase activity decreased till the appearance of shoots.