Department of Botany, Raja Balwant Singh College, Agra-282 002, India
*Corresponding author's present address: 1/104-A, Kaley Ka Tal, Delhi Gate, Agra-282 002.
A protocol for micropropagation of Ziziphus jujuba was developed by using nodal explants in different media compositions. Various morphogenic responses of explants have been observed by using different media, viz. MS, B5 and N6. MS basal medium was found to be the best among them for shoot proliferation (93.75%). Shoot development was initiated within a week. No callus development was observed. Inorder to enhance the shoot regenerative potential, explants were cultured on MS medium supplemented with various cytokinins, viz. BAP, kinetin and TDZ. MS medium supplemented with BAP (1.78 μM) was found to be ideal for shoot development. Among various auxins used, 2.69 μM NAA proved to be the best to induce healthy roots with minimum callused thickening, resulting in maximum survival of plants (80%) in the field.
Ber, micropropagation, nodal shoots, Ziziphus jujuba
