Indian Journal of Plant Physiology

Two genotypes of wheat (Triticum aestivum L.), a salt tolerant KRL 1–4 and a salt sensitive HD 2329 were subjected to stress treatments of control, 10 mM B, 120 mM NaCl and 10 mM B+ 120 mM NaCl at the seedling stage in a BOD incubator at 26±1°C for 96 h. Proteins from coleoptile and radicle were resolved by SDS-PAGE. In tolerant cultivar (KRL 1–4), two proteins of 83.6 and 28.1 kDa appeared in the coleoptile and a 35 kDa protein appeared in the radicle in the seedlings treated with B and B+NaCl. Similarly, 46.6 kDa protein was observed in the radicle of KRL 1–4 only in B treated seedlings. These results clearly indicate characteristically B induced proteins. Two proteins of 74.2 and 48.8 kDa in HD 2329 coleoptile and a 64.6 kDa protein in the radicle of both KRL 1–4 and HD 2329 seedlings were seen specific to salt treatment and were absent in boron treated seedlings. Likewise, a 70 kDa protein in the coleoptile profile of KRL 1–4 seedlings was expressed only with salt treatment, but was absent in boron treatment.