Multiple shoot and plant regeneration from immature leaflets of in vitro origin in curryleaf (Murraya koenigii Spreng)
Abstract
High frequency shoot regeneration protocol from in vitro raised young leaves of Murraya koenigii Spreng was standardized. A combination of 6.6 μM 6-benzyl amino purine (BAP) and 2.9 μM indole 3-acetic acid (IAA) has induced significantly (p<0.05) more number of shoots per explant (21.5). Maximum length (2.72 cm) of regenerated shoots (8 weeks after initial culture) was observed on Murashige and Skoog (MS) medium with 5.5 μM BAP and 2.9 μM IAA and on medium with 4.4 μM BAP and 2.9 μM IAA (2.69) which also gave maximum number of leaves per shoot (13.4). Maximum number of roots (recorded 6 and 8 weeks after initial culture) from cut end of induced shoots was obtained on MS medium supplemented with 26.9 or 37.6 μM α-naphthalene acetic acid (NAA). Percentage seedling establishment and whole plant fresh and dry weight were best on hardening medium containing peat, perlite and sand at 1: 1: 2 ratios.
Keywords
Cytokinin, hardening, micropropagation, plant regeneration, tissue culture
