Establishment of shoot cultures and plant regeneration of Passiflora edulis Sims

Passiflora edulis Sims, a woody climber belonging to the family passifloraceae has been micropropagated successfully by culturing nodal and internodal segments. The explants were cultured in Murashige and Skoog (MS) basal medium augmented with 6-benzylaminopurine. Maximum percentage of shoot proliferation was achieved in MS basal medium fortified with 6-benzylaminopurine (4.44 mM). The maximum percentage of callus formation was achieved on MS medium augmented with 2, 4-dichlorophenoxy acetic acid (4.52 mM). Maximum percentage of shoot proliferation from the internodal derived calli was achieved on MS medium supplemented with 6-benzylaminopurine (2.22 μM and 4.44 mM). The in vitro raised shootlets were transferred to MS medium fortified with indole butyric acid (IBA) and highest percentage of rooting was observed in IBA-4.90 mM.