Ex situ culture studies on a medicinal plant Pedalium murex (Linn)

In the present study, nodal, internodal segments of Pedalium murex (Linn) were cultured on Murashige and Skoog (MS) medium supplemented with 3 mg/l 2,4-D, which was found to be most suitable for the successful callus initiation. The callus was then cultured on MS medium consisting of MS basal salts, 0.8% agar and 3% sucrose supplemented with BAP with a concentration of 3 mg/l, which lead to the highest rate of multiple shoot bud initiation. The in vitro shootlets were cultured on different concentrations of 2, 4-D in combination with BAP in the culture medium. For flower initiation, the highest rate of flower initiation was in the culture media supplemented with 1.5 mg/l 2,4-D and 1.5 mg/l BAP.