1A.I.C.R.P. on Niger, Zonal Agril. Research Station, Igatpuri, Dist. Nashik-422 403, (M.S.)
2Department of Agricultural Botany, Dr. B.S.K.K.V., Dapoli, Dist. Ratnagiri-415 712, (M.S.)
* Corresponding author, E-mail: yoga_ban@yahoo.co.in
Efficacy of certain physico-chemical characters of grain and electrophoretic method (SDS-PAGE) was tested in rice varietal identification. SDS-PAGE of ten rice varieties resulted in scoring of twenty polymorphic bands. Out of these distinct presence/absence was exhibited by band no. 2 (Rm 0.23), 3 (Rm 0.26), 5 (Rm0.30), 6 (Rm 0.32), 7 (Rm 0.34), 8 (Rm 0.35), 12 (Rm 0.44) and 23 (Rm 0.97). The varieties were distinguishable from each other by determining the presence and/or absence of specific protein bands and their intensity in electrophoregrams. Polypeptide polymorphism along with ‘seed keys’ may be used for differentiation and identification of rice cultivars and for utilization in a rice varietal improvement programme.
Protein marker, rice, SDS-PAGE, seed keys, varietal identification
