Multiple shoot induction in intact shoot tip, excised shoot tip and nodal segment explants of Rauwolfia Serpentina

The effect of different plant growth regulators alone or in combination on multiple shoots production from different explants of Rauwolfia serpentina L. was studied. Multiple shoots were obtained from intact or excised shoot tip, and nodal segment in MS medium supplemented with BAP (2.0 mg l⁻¹) and NAA (0.5 mg l⁻¹). Maximum number of shoots (16–24 per explant) was obtained from the excised shoot tips.