In vitro propagation of Ormocarpum sennoides (Willd) DC. Prodr. from shoot tip explant

Ormocarpum sennoides, a medicinal tree has been successfully micropropagated using shoot tip explant on Murashige and Skoog (MS) basal medium and MS medium with different concentrations (0.5, 1.0, 2.0, 3.0 and 4.0 mg l⁻¹) of benzyladenine (BA), adenine sulphate (Ads) and kinetin (Kn). A maximum of 10 shoots were observed at 3.0 mg l⁻¹ BA. The regenerated shoots were transferred to MS medium fortified with different concentrations (0.5 – 2.0 mg l⁻¹) of gibberellic acid (GA₃) and 3.0 mg l⁻¹ BA for shoot elongation. The maximum of 2.0 – 2.5 cm long shoots were observed at 1.0 mg l¹ GA₃. The regenerated shoots were excised and transferred to rooting medium containing indole acetic acid (IAA), -naphthalene acetic acid (NAA) and 3-indole butyric acid (IBA) at different concentrations. The highest of 60% of rooting was observed at 0.3 mg l⁻¹ IAA, IBA and NAA. The in vitro derived plantlets were hardened and acclimatized in soil.