Dynamic peptide profiles of germinating mungbean: In relation to their nature and separation pattern

Low molecular weight peptides (3 KDa to 0.5 KDa) from different germinating phases of mung bean [Vigna radiata (L) Wilczek. cv. Sonali B1] were purified through ion exchange, ultrafiltration and sephadex gel permeation chromatography. The peptides were subjected to paper chromatographic and capillary electrophoretic separation, for the determination of dynamic and temporal manifestation of their occurrence. Analysis of peptides revealed mainly two groups, one of which is constitutive throughout while the other mainly appeared after 24 hrs and disappeared at 5 days of post germination phases. Amino acid analysis of these peptides indicated adequate similarities with legume storage protein. Lack of cysteine and methionine in peptide pool indicated that they are active in all different shapes suitable for transport and absorption.