1Natural Products Department, Institute of Minerals & Materials Technology (C.S.I.R.), Bhubaneswar-751 013.
2School of Life Sciences, Sambalpur University, Sambalpur-768 019.
*Corresponding author, E-mail: mtarasu@yahoo.com
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A rapid and efficient in vitro propagation protocol for Ruta graveolens L. from nodal explants was developed. Multiple shoot formation was induced from nodal segments on Murashige and Skoog (MS) medium with 4.4–17.6 M−1 6-benzyl adenine (BA), 1.13–9.06 M−1 thidiazuron (TDZ). Maximum number of shoots were induced (58.0±1.51) with 4.4 M BA and 1.42 M indole-3-acetic acid (IAA). Shoot differentiation occurred directly from the cut ends without any callus formation. In vitro regenerated shoots rooted best on half-strength MS medium containing 1.22 M indole-3-butyric acid (IBA). Plants with a well developed root system could be successfully established under in vivo condition, where the survival rate was 90 per cent.
Growth regulators, nodal explants, Ruta graveolens, shoot regeneration
