Indian Journal of Plant Physiology

  • Year: 2008
  • Volume: 13
  • Issue: 2

Characterization of endosperm protein from different varieties of wheat isolated by triton X-114

  • Author:
  • Vinod Kumar Dhatwalia1,†, O.P. Sati1, M.K. Tripathi2, Versha Rai1
  • Total Page Count: 3
  • DOI:
  • Page Number: 195 to 197

1Department of Chemistry, H.N.B. Garhwal University, Srinagar, Uttarakhand.

2Department of Biotechnology, ICFAI University, Dehradun, Uttarakhand.

*Corresponding author, E-mail: vinodguru07@yahoo.com

†Present address: Upper Sodhowala (near Dolphin Institute), P.O. Manduwala, Dehradun

Abstract

The wheat (Triticum aestivum) is one of the most abundant sources of energy and protein for world's population. A new basic protein has been Isolated from wheat endosperm by Triton X-114 phase partitioning. This protein isolated from wheat endosperm has been named puroindoline. The puroindoline, endosperm-specific proteins involved in wheat seed hardness, are small proteins reported to have in vitro antimicrobial properties. Antimicrobial peptides play a role in the immune systems of animals and plants by limiting pathogen infection and growth. Puroindoline may also be a membranotoxin that might play a role in the defense mechanism of plants against microbial pathogens.This protein is isolated and characterized from eight different varieties of Indian wheat.It is concluded that endosperm protein profiles could be useful markers for studying diversity and classification of adapted cultivars.

Keywords

Endosperm, puroindoline, triton X-114, wheat