Plant Biotechnology Laboratory, Natural Products Department, Institute of Minerals and Materials Technology (CSIR), Bhubaneswar-751 013, India.
*Corresponding author: E-mail: mtarasu@yahoo.com
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A regeneration protocol has been developed for Gmelina arborea Roxb. using cotyledonary nodes. Multiple shoots were induced on Murashige and Skoog's (MS) medium supplemented with 6-benzyladenine (BA) and thidiazuron (TDZ) either alone or in combination with either indole-3-acetic acid (IAA) or α-napthalene acetic acid (NAA). Cotyledonary nodes cultured on medium with BA (4.4 μM) + IAA (1.4 μM) produced higher number of shoots. Cultures maintained in TDZ supplemented medium showed poor response. In vitro regenerated shoots were cultured on to root induction medium consisting of half-strength MS supplemented with IAA or indole-3-butyric acid (IBA) either alone or in combination with 2, 3, 5-triiodobenzoic acid (TIBA). Rooting was best in the medium supplemented with 4.9 μM IBA + 2.0 μM TIBA. Rooted plantlets were acclimatized and transferred to the field with 70% survival rate.
BA, cotyledonary node, Gmelina arborea, IBA, micropropagation
