Department of Biosciences, Saurashtra University, Rajkot-360 005, Gujarat, India.
*Corresponding author: E-mail: vsthaker@hotmail.com
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Multiple shoots were induced from nodal region of carnation (Dianthus caryophyllus L.) in Murashige and Skoog medium containing 1.5 mg l−1 BAP and 0.5 mg l−1 NAA. Induction of the shoots was observed in three weeks, but good amount of tissue turned brown in sub-cultures. Cationic and anionic peroxidase activities were studied in green and brown multiple shoot culture of carnation. It was observed that anionic protein was more in brown tissue and specific anionic peroxidase activity was also higher. It is concluded that anionic peroxidase plays an important role in browning of the tissue.
Anionic peroxidase, carnation, cationic peroxidase, multiple shoots
