Pakistan Council of Scientific and Industrial Research, Laboratories Complex, Shahrah-e-Dr. Salimuzzaman Siddiqui, Off University Road, Karachi-75280, Pakistan.
*Corresponding author, E-mail: fatima_bi220@hotmail.com
The elicitor activity of high molecular weight polysaccharides (HMWP) of Hypnea musciformis (red algae) of Karachi coast was determined and established in chickpea tissues. A simple extraction procedure and HPLC method was developed to analyze the major and minor components of induced phytoalexins. LC-Electrospray-mass spectrometry, characterized some of the individual components and as a result, four compounds (isoflavonoids) and their glycoconjugates likewise formononetin, maackiain, naringin and naringin melonate were identified by their mass spectra.
Elicitor, Hypnea musciformis, isoflavonoids, phytoalexin
