Plant regeneration through organogenesis from young leaves of Chlorophytum borivilianum

Plant regeneration through organogenesis was standardized from in vitro regenerated leaves of safed musli. Callus induced from the leaves within two weeks of culture on MS media with various concentrations of 2,4-D (0.25 to 4.0 mg/l) alone and in combination with other auxins and cytokinins. Among all, 1.5 mg/l 2,4-D resulted in embryogenic callus from the leaves. However, further differentiation of callus was not observed on 2,4-D media. Embryogenic calli were transferred to MS medium with various concentrations of BAP (0.5 to 3.0 mg/l) along with two different concentrations of Kn (0.25 and 0.5 mg/l). The frequency of shoot bud primordia production varied from 31.25 to 58.32% in different concentrations of BAP and Kn. Among all, high frequency of plant regeneration (83.3%) was achieved on MS with 1 mg/l BAP + 0.5 mg/Kn. Higher concentration of BAP induced more number of shoot buds, but many of them failed to regenerate. Regenerated shoots were rooted on half strength MS media with different concentrations of IAA and IBA. High frequency rooting (73.3%) was observed on half strength MS with 2 mg/l IBA. Rooted plantlets were transferred to pots and established in the field with 90% survival rate.