Genetic diversity analysis of elite parental lines of cotton using RAPD, ISSR and isozyme markers

The genetic diversity and similarity were investigated in nine elite parental lines of cotton using 25 RAPD, 3 ISSR and 2 isozyme markers. Total 189 amplicons were generated using all 30 markers, of which 117 bands were found to be polymorphic. Highest polymorphism was seen with ISSR primers (66.66%) while, it was least (42.85%) with the isozymes. Based on Jaccard's similarity coefficient, the similarity index was in the range of 0.11 to 1.00 among nine parental lines. The lowest similarity (11%) was found among LRA-5166, GCot.10 and 76 IH-20. The dendrogram of cotton lines showed two major clusters. The Parental line LRA-5166 was found in one cluster and the rest eight parental lines were found in different sub-sub cluster of another cluster. The results suggested that the genetic constituents of LRA-5166 is quite different than other tested parental lines.