Indian Journal of Plant Physiology

  • Year: 2009
  • Volume: 14
  • Issue: 2

An efficient method of micropropagation of ginger (Zingiber officinale Rosc. cv. Supravaand Suruchi) through in vitro rhizome bud culture

  • Author:
  • Kambaska Kumer Behera, Santilata Sahoo
  • Total Page Count: 7
  • DOI:
  • Page Number: 162 to 168

P.G. Deptt. of Botany, Utkal University, Vanivihar, Bhubaneswar, Orissa, India.

* Corresponding author, E-mail: kambaska@yahoo.co.in

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Abstract

An efficient, simple micropropagation method was developed for Zingiber officinale Rosc. (cv, Suprava and Suruchi) using fresh rhizome sprouting bud in semisolid culture media. Explants were cultured on Murashige and Skoog's (MS) medium supplemented with different concentrations and combinations of BAP (6-benzyl- amino-purine) and NAA (− naphthalene acetic acid) for shoot and root induction. Explants cultured on MS basal medium supplemented with 2.0 mg/l BAP + 0.5mg/l NAA showed highest rate of shoot multiplication. In vitro shoots were rooted on to the half strength MS basal medium supplemented with 2.0 mg/l NAA and rooting was better. Rooted shoots were transplanted in the green house for hardening and their survival was 95% in the field condition.

Keywords

Growth regulators, micropropagation, rhizome sprouting bud, tissue culture, Zingiber officinale