1P.G. Deptt. of Botany, Utkal University, Vanivihar, Bhubaneswar, Orissa, India.
2P.N. College (Autonomous), Khurda, Orissa, India.
*Corresponding author, E-mail: kambaska@yahoo.co.in
**Present address: c/o Prof. S. Chattopadhyay, Department of Biotechnology, National Institute of Technology, Dungapur, Mahatma Gandhi Avenue, Dungapur-713 209, West Bengal, India.
Nodal vine segments from 45 days old plants of Dioscorea alata L. (cv. Hinjilicatu) were cultured on Murashige and Skoog's (MS) medium supplemented with different concentration and combinations of BAP and Kn along with NAA. Explants cultured in MS basal medium supplemented with 2.0 mg/l Kn +1.0 mg BAP + 0.5 mg/l NAA showed highest rate of shoot multiplication. When in vitro shoots were inoculated on to the half-strength MS basal media supplemented with 2.0 mg/l NAA and rooting was more profuse. Rooted shoots were transplanted in the green house for hardening and their survival was 90% in the field condition without any morphological variation.
Auxin, cytokinin, Dioscorea, micropropagation, nodal vine cuttings
