Indian Journal of Plant Physiology

  • Year: 2009
  • Volume: 14
  • Issue: 3

In vitro flowering in hill maize: A Novel Technique for future

  • Author:
  • Rohit Joshi1, Alok Shukla1, Pramod Kumar2,
  • Total Page Count: 4
  • DOI:
  • Page Number: 299 to 302

1Department of Plant Physiology, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand-263 145.

2Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi-110 012.

Abstract

In vitro flowering through shoot tip culture was analyzed in four maize genotypes, viz. Vivek-4, Vivek-5, VL-42 & Him-129. Plants were regenerated from first nodal region of shoot tip in MS medium supplemented with 2 mg/l BAP and 500 mg/l casein hydrolysate. Multiple shooting was observed in all these varieties. After six week (42 days) of culture miniature cobs were developed only in three genotypes, i.e. Vivek-5, VL-42 and Him-129, under 16 hr photoperiod and 150 mol m−2 sec−1 light intensity at 25±2°C. In vitro flowering may be used as an effective tool for advancing hybrid production and to study the physiological and molecular aspects of flowering in maize.

Keywords

In vitro flowering, shoot tip culture, Zea mays