Indian Journal of Plant Physiology
  • Year: 2009
  • Volume: 14
  • Issue: 3

Isolation of differentially regulated partial cdna with respect to water stress from rice

  • Author:
  • Vinita Kumari1, Vandana Tyagi2, Kishwar Ali1, Aruna Tyagi1,
  • Total Page Count: 4
  • Page Number: 306 to 309

1Division of Bichemistry, Indian Agricultural Research Institute, New Delhi-110 012.

2National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi-110 012.

*Corresponding author, E-mail: at_bio@iari.res.in

Abstract

In the present study a partial cDNA sequence of 308 bp showing induction under water stress was isolated from rice cv. Nagina-22 (N22) and sequenced. The sequence has 150 bp 5′ untranslated region and the 156 bp coding sequence. Coding sequence has ATG initiation codon and encodes 52 amino acids. On homology search analysis, it was observed that the coding sequence had 100% homology with protein phosphatase 2C gene of Arabidopsis thaliana and corresponds to N terminal region of the gene which has very important role in ABA signaling. Protein phosphatase 2C negatively regulates ABA response under water deficit stress.

Keywords

ABA, water stress