Indian Journal of Plant Physiology

  • Year: 2010
  • Volume: 15
  • Issue: 1

Regeneration of Liquorice (Glycyrrhiza Glabra L.) from Cultured Nodal Segments

  • Author:
  • P. Sharma1, M.K. Tripathi1, G. Tiwari2,, S. Tiwari3, B.S. Baghel4
  • Total Page Count: 10
  • DOI:
  • Page Number: 1 to 10

1Horticultural Biotechnology Laboratory, J.N. Agricultural University, Jabalpur-482 004, India.

2Department of Medicinal & Aromatic Plants, J.N. Agricultural University, Jabalpur-482 004, India.

3Division of Tissue Culture & Transgenics, Biotechnology Centre, J.N. Agricultural University, Jabalpur-482 004, India.

4KNK-College of Hoticulture, Mandsaur-458 001.

*Corresponding author, E-mail: tiwarig_111@yahoo.co.in.

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Abstract

A protocol has been established to propagate local cultivar of Glycyrrhiza glabra (L.) through indirect and direct in vitro organogenesis from nodal segment explants excised from 4–5 months-old plants. In the present study, induction medium B52D (B5 + 2.0 mgl−1 2, 4 D + 20.0 gl−1 sucrose + 7.5 gl−1 agar) induced callusing in higher frequencies (65.93%). Culture medium B5.5B.5N (B5 + 0.5 mgl−1 BA + 0.5 mgl−1 NAA + 20.0 gl−1 sucrose + 7.5 gl−1 agar) was found to be more responsive for shoot proliferation (94.12%), shoots per explant (9.32) and mean shoot length (5.20 cm). Among rooting media B5.5I (B5 + 0.5 mgl−1 IBA+ 10.0 gl−1 sucrose + 7.5 gl−1 agar) proved to be superior for higher root proliferation (84.92%) and mean root length (2.13 cm). Regenerated plantlets were established successfully in the field after hardening.

Keywords

Glycyrrhiza glabra, nodal segment culture, organogenesis, plantlet regeneration