1Department of Botany, Yogi Vemana University, Vemanapuram, Kadapa-516 003, Andhra Pradesh, India.
2Departmento Senfimizo, Faculta di Agararia, Universita degli Studi di Palerma, Viale delle Scienze11, 90128, Palermo, Italy.
3Biotechnology Research Centre, Tirupati, Andhra Pradesh, India.
4Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, 35, Keyan Road, Zhunan Town, Miaoli County 350, Taiwan ROC.
*Corresponding author, E-mail:pssvkhan@yahoo.com.
*Present address: Prof. M.A. Germana, Departmento SENFIMIZO, Faculta di Agararia, Universita degli Studi di Palerma, Viale delle Scienze11, 90128, Palermo, Italy.
The current experiments were undertaken to study the effect of thiadiazuron (TDZ) and ascorbic acid (AA) on recurrent production of plantlets from seeds of Syzygium alternifolium (Wight.) Walp. The multiple shoots were induced from embryonal axes of seeds cultured on basic culture medium (BCM) containing half strength Murashige and Skoog (MS) salts, B5 vitamins, 2 mg/l glycine, 2% (w/v) sucrose, 0.8 % (w/v) agar, 1.0 mg/l TDZ and AA (1000 mg/l). Our findings suggested recurrent production of plantlets and their hardening process to obtain 70% survival rates. The tissue culture protocol can be used for en masse propagation and conservation of this important multipurpose fruit tree.
Ascorbic acid, in vitro, Myrtaceae, polyembryony, Syzygium, TDZ
