*Corresponding author, E-mail: romiarora@yahoo.com.
1These authors equally contributed to this work
Protocol for micropropagation of Gladiolus grandiflora, an economically important horticultural crop has been developed using aseptically grown cormel slices as explants. Better proliferation and multiplication of calli were obtained on Murashige and Skoog (MS) basal medium supplemented with 2.0 mgl−1 NAA and 1.0 mgl−1 BAP. Regenerable calli were formed from the basal region of in vitro cultured cormel slices in approximately three weeks after explants were placed on callus induction medium. For most prolific shoot induction one gram (fresh weight) of callus was placed on shoot induction medium containing MS basal salt and 0.5 mgl−1 BAP. Shoot formation occurred one month after inoculation of callus on shoot induction medium. The standardized regeneration protocol will help to impart desirable traits in Gladiolus cultivar ‘Snow Princess’ for further transformation and genetic modification.
Callus, cormel slice, gladiolus, regeneration
