Regeneration of sour cherry (Prunus ceraseus) through In-vitro propagation

Dormant cutting procured from mature plants, were subjected to forcing treatment for in-vitro propagation. The culture media i.e., Murashige and Skoog medium (MS) with three strength levels viz., full, half and three fourth were used. Plant growth regulators viz. BAP (0.25 mg1⁻¹, 0.50 mg1⁻¹, 0.75 mg1⁻¹) and IBA (0.00 mg1⁻¹ and 0.01 mg1⁻¹) were supplemented with MS media. The design of experiment was CRD with three replications. MS half strength basal medium supplemented with 0.50 mg l⁻¹ BAP plus 0.01 mg l⁻¹ IBA resulted in the highest survival percentage of in vitro cultures. MS medium supplemented with 1 mg l⁻¹ BAP + 0.10 mg l⁻¹ IBA fortified with 2 mg l⁻¹ BAP + 0.10 mg l⁻¹ IBA recorded the maximum proliferation efficiency. MS medium supplemented with BAP @ 0.1 mg l⁻¹ and devoid of auxin recorded the optimum elongation of micro shoots. MS medium fortified with 2 mg l⁻¹ IBA gave the highest rooting percentage and average number of roots per explant. The studies culminated in standardization of protocol for in vitro propagation of sour cherry rootstock.