1Laboratory of Plant Morphogenesis, Department of Plant Cytology and Cytochemistry, Institute of Plant Physiology, Cytology and Cytogenetic, University of Lódz, 90–237 Lódz, Poland
2Laboratory of Tissue Culture, Department of Biotechnology and Cytochemistry, Plant Breeding and Acclimatization Institute, Radzików 05–870 Blonie, Poland.
*Corresponding author, E-mail: bartek_adamczyk_79@o2.pl.
The aim of this paper was to study the interaction between protein and inorganic nitrogen use by Triticum aestivum seedlings cultivated on different variants of the Murashige-Skoog medium (MS): i) standard MS, ii) without inorganic nitrogen with 0.1% casein, iii) with 0.1% casein and inorganic nitrogen (5%, 25% or 100% of inorganic nitrogen from standard MS medium). The highest proteolytic activity in the medium and growth of wheat seedlings were obtained on the medium with casein and inorganic nitrogen. We assume that proteases secreted from wheat roots digested casein producing a pool of easy-accessible organic nitrogen and simultaneously applied inorganic nitrogen provided even higher biomass. Our studies suggest that the highest wheat yield can be obtained by usage of inorganic and organic nitrogen in adequate proportion.
Plant nitrogen nutrition, protease exudation, Triticum aestivum
