Indian Journal of Plant Physiology

  • Year: 2010
  • Volume: 15
  • Issue: 2

Direct rhizogenesis from in vitro leaves of withania somnifera (l.) dunal

  • Author:
  • K. Mohamed Rafi, A. Aslam, S. Kohila1, J. Tanweer, A. Shajahan
  • Total Page Count: 4
  • DOI:
  • Page Number: 173 to 176

1Department of Biotechnology, Mother Terasa Women's University, Attuvampatty Campus, Kodaikanal, Tamil Nadu.

PG & Research Department of Botany, Jamal Mohamed College, Tiruchirappali-620 020, Tamil Nadu.

*Corresponding author, E-mail: shajahan.jmc@gmail.com.

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Abstract

Direct in vitro rhizogenesis was induced in Withania somnifera (L.) Dunal leaf segments using exogenous addition of auxins. Among the four types of explants (internodal, nodal, young leaf and mature leaf segments), young leaves responded better and formed roots in the midrib region on the contact surface when placed on Murashige and Skoog's (MS) basal medium containing various types (IAA, IBA and NAA) and concentrations of auxins. The strength of the MS media (1/4, 1/2, 3/4 and fullstrength) treatments had apparent effect on rooting. Maximum rooting in young leaf (95%) occurred in ½ strength MS + IAA medium. The other types of auxins were good for inducing root in other explants. Only 20 percent of the cultures produced roots if explants were grown on full-strength MS medium supplemented with IBA.

Keywords

Auxins, rhizogenesis, Withania somnifera