Studies on In vitro regeneration and direct organogenesis in pea (Pisum sativum L.)

A regeneration protocol has been optimized in Pea (Pisum sativum L.) genotypes HUDP-15 and IPF-99-25 with explants viz. embryonic axes as such, and also as sliced and decapitated and cotyledonary node. Direct shoot regeneration was achieved on medium containing MS salts + B₅ vitamins and various concentrations of BAP, NAA and IBA. The best direct regeneration efficiency (10.37±3.18 shoots/explant) and frequency (84.66±0.84%) was achieved from embryonic axes slices on MS salts + B₅ vitamins + 3.0 mg/l BAP + 2.0 mg/l IBA + 40 gm/l sucrose. The regenerated shoots were excised and transferred to rooting medium, containing NAA and IAA at various concentrations. The highest rooting was observed on the medium supplemented with ½ MS salts + B₅ vitamins + 2.0 mg/l IAA + 30 gm/l sucrose, rooted plants were acclimatized and transferred to field with good survival rate.