Laboratory of Plant Tissue Culture and Stress Physiology, Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi.
* Corresponding author, E-mail: pdwivedi25@rediffmail.com
An efficient in vitro shoot multiplication protocol was developed for Gymnema sylvestre R.Br. which is a potent anti-diabetic plant. Nodal explants were inoculated in half strength MS media containing different auxins (IAA and IBA) and cytokinins (BAP and Kinetin) at various concentrations for rapid multiple shoot induction. Multiple shooting (3.25±0.62) was obtained in presence of BAP and Kn at 3.0 mg/l each. Best response in terms of shoot length was recorded (1.97±0.06 cm) in the media supplemented with Kn and IAA at 1.5 mg/l each. Microshoots obtained in vitro were inoculated in ¼ MS media which produced maximum of 5 roots at 1.0 mg/l IBA.
Anti-diabetic plant, In vitro multiplication, Gymnema sylvestre, Gymnemic acid
