Molecular analysis of maize hybrids and their parents using issr and protein profiling

PCR-based Inter Simple Sequence Repeat (ISSR) markers and protein profiling on SDS-PAGE were employed to assess genetic diversity in four maize hybrids and their parents. The DNA was isolated from CTAB extraction method. In ISSR analysis 3 primers gave amplified products. They gave 35 scorable fragments with 100 percent polymorphism and gave 11.67 polymorphic bands per primer. ISSR profiling similarity matrix was obtained using Jaccard Similarity Coefficient was observed up to 0.75 and on this basis a dendrogram was constructed with UPGMA method. The soluble protein was extracted from maize seeds using 0.2 M phosphate buffer (pH 7.4). The concentration of soluble proteins ranged between 20.0 to 90.0 mg/g of seeds. For protein profiling 10% and 15% polyacrylamide gels were used to resolve polypeptides through SDS-PAGE. 24 scorable bands were resolved on 10% gel of which 22 bands were polymorphic (91.67%) and 19 scorable bands were resolved on 15% gel in which 17 bands were polymorphic (89.47%). In protein profiling Jaccard Similarity Coefficient lie between 0.21 to 0.65. According to the dendrogram of ISSR and protein profiling, hybrids resembled more with their female parent.