Identification of markers for NaCl tolerance in cell lines of Jatropha curcus- A bio-diesel plant

Sub-culturing of leaf callus on Murashige and Skoog's (MS) medium with 2.5 x 10 ⁶M á-naphthalene acetic acid (NAA) and 5 x 10⁻⁶M each of 6-benzylaminopurine (BAP) and kinetin (KIN) for six generations supplemented with NaCl (105mM) or without NaCl yielded six different callus lines viz. control, unadapted salt stressed and salt adapted. Adapted calluses were first shifted to NaCl free medium (adapted →C) and later to salt supplemented medium (adapted →C→S). SDS-PAGE analysis revealed that polypeptides of MW 1000, 79.4 and 67.6 kDa, synthesized in response to NaCl are critical for survival of cells under salt stress. RAPD analysis of salt exposed variants showed that primers 100C04, 90B11 and BG56S110C15 can possibly be employed to detect amplification products related to salt tolerance in Jatropha.