Agrobacterium Mediated in planta Transformation of Gossypium hirsutum Cv. G. Cot. 10

An improved protocol of using simple in planta transformation methods (Injection method and Cut method) were developed for Gossypium hirsutum. The frequency of transformation was 1.16% for injection method and 0.83% for cut method. The effect of different factors like age of explants, pH of co-cultivation medium, effect of kanamycin and co-cultivation time period of Agrobacterium tumefaciens on the efficiency of regeneration and transformation was studied. The combined result of this study shows that three days old seedlings inoculated with Agrobacterium and co-cultivated for three days in half strength MS medium at pH 5.8 had highest frequency of transformation.