In Vitro Regeneration of Oncidium Gower Ramsey by High Frequency Protocorm Like Bodies Proliferation
Abstract
A commercially viable procedure for high frequency in vitro plantlet regeneration of Oncidium Gower Remsey was established using defined and undefined substitutes. Various orchid micropropagation media were used to study protocorm like bodies mediated plantlet differentiation from shoot tips. Protocorm like body formation and proliferation was best seen of Vacin and Went (1949) medium with the addition of 15% coconut milk. Whereas, plantlet differentiation took place on Murashige and Skoog (1962) medium supplemented with 200 mg 1−1 casein hydrolysate. The regeneration process from shoot tip to ex-agar plants took seven months. Proliferation of protocorm like bodies (PLB) at a multiplication factor of five is a continuous process for the last two years.
