Multiple shoots were regenerated from seedling shoot tips of nine cultivars of sesame (Sesamum indicum) and one wild species, S. occidentalis. The percentage response of multiple shoot induction varied from 38 to 100 in Murashige and Skoog's, Linsmaier and Skoog's, Gamborg's and L6 media. Total number of shoots regenerated in each inedium/explant and mean number of shoots/explant varied significantly. The total number of shoots recovered in each medium and mean number of shoots/explant improved remarkably, consequent to the pretreatment of seedling with GA3 (8 mg/1) and BAP (8 mg/1) with varying frequency (85 to 100%) in different genotypes. Elongated shoots were rooted on MS and L6 media fortified with 0.1 mg/1 to 0.3 mg/1 NAA plus 3% sucrose. Rooting ranged from 65 to 100% in different treatments and genotypes. Whole plants were transferred successfully to the soil with 60% survival rate.
