Cycloheximide (Cyc), actinomycin-D (Act-D), bromouracil (BrU) and fluorour-acil (FU) all enhance rooting when applied continually at low concentartions and more so when given as a pretreatment for 6 or 24 hr and subsequently transferred to IAA in light-raised hypocotyl cuttings of Phaseolus mungo and Impatiens balsamina. In dark grown cuttings with excised leaves and apex, these stimulated rooting when the cuttings were transferred after pretreatment with antimetabolites to sucrose or IAA+ sucrose. These also hastened the GA3-caused floral bud initiation and increased the number of floral buds in 1. balsamina.
Inhibitors of oxidative phosphorylation like DNP, sodium azide and ammonium sulphate also stimulated rooting.
