Uptake of sulphur and iron and their partitioning into leaf (blade and sheath), stalk and roots was studied in sugarcane settlings raised from top cane buds and stubble buds The settlings raised from top cane buds assimilated relatively more sulphur compared to the ones raised from stubble buds. However, the rate of sulphur and iron uptake was relatively more in the settlings raised from stubble buds, A comparison of the shoot: root ratio indicated that in the settlings raised from stubble buds, dry matter production per unit root weight was relatively more.