Immobilization of acid phosphatases of sugarcane juice on soil has been studied using acid activated cane field soil as support. The soil immobilized enzyme system was able to release phosphorus from tricalcium phosphate (TCP) continuously for about 10 days at 30°C and it showed full activity in three cycles. Increasing surface area of immobilized soil by spreading during enzyme reaction at a moisture level of 55% greatly increased the enzyme activity.
