Pot culture experiments were conducted to study the activities of nitrate reductase, glutamate dehydrogenase and glutamine synthetase at different stages of growth and development of chickpea. Different pattern of enzyme activity was noted in the leaves and nodules. In leaves, highest level of nitrate reductase activity was observed at the vegetative stage, for glutamine synthetase the activity was maximum at flowering and peak values for glutamate dehydrogenase were noted at pod formation stage. In nodules, maximum activity for both glutamine synthetase and glutamate dehydrogenase was observed at flowering which declined sharply with the advancement in age. Application of phosphorus at the rate of 60 kg P2O5/ha increased the activity of NR and GDH, while lower dose (30 kg P2O5/ha) was most favourable for GS activity. Phosphorus at 80 kg P2O5/ha, was detrimental for the enzyme activities.
