Tissue culture Laboratory, The International Rice Research Institute P.O. Box 933, Manila, Philippines
1 Present address: Plant Physiology Division, Bangladesh Rice Research Institute, Joydebpur, Gazipur, Bangladesh
Anther culture of rice provides a useful tool for rapid regeneration of homozygous plants. Rice haploids generated through tissue culture are usually produced by a two-step procedure involving callus induction and then plantlet regeneration. In the present study, one-step procedure was worked out where plants could be regenerated in the same plates without transferring to a separate medium. Two media which contained- napthaleneacetic acid (NAA) was compared to Gamborg’s (BS) medium containing 2, 4-D. Modified potato medium (P2) was similar to B5 in callus induction efficiency. A new medium (M10) produced significantly higher number of direct plantlets as well as green plantlets without transferring the calli to differentiation medium. Modified potato medium gave the highest number of albino plantlet and roots. The directly regenerated plants were ready to be transferred to culture solution within 7-8 weeks.
