Department of Botany, North-Eastern Hill University Shillong, 793014
Excised shoot apices of Dendrobium wardianum, cultured on MS medium supplemented with inorganic and organic nitrogenous sources, viz., calcium nitrate, ammonium sulphate, urea and amino acids (alanine, glutamine, glycine, leucine and serine) showed varied responses. Calcium nitrate and urea gave rise to direct multiple shooting besides forming protocorm-like bodies. Regenerants obtained by rooting isolated shoots and germinating protocorm-like bodies were successfully transplanted to a substratum containing charcoal pieces, brick bats and coconut fibres. The rate of survival was recorded to be 65% under glass house conditions.
