Influence of stomatal frequency and stomatal aperture in young, mature and old leaves of species belonging to both dicotyledonous, viz., Vigna catjang, Phaseolus mango, Cammelina benghalensis. Impatiens balsamina, Cestrum noeturnum and monocotyledonous group Typhonium trilobatum was studied after 6 days of incubation in light and dark. It was concluded that stomatal frequency as well as stomatal pore were not consistantly interrelated with the senescence of intact leaves.