Indian Journal of Plant Physiology
  • Year: 1993
  • Volume: 36o
  • Issue: 1

Inhibition of Nitrate Reductase Activity by Mercury in Bean Leaf Segements

  • Author:
  • Jot Vyas, Rekha M Puranik1
  • Total Page Count: 4
  • Page Number: 57 to 60

School of Biochemistry, Devi Ahilya Vishwavidyalaya, Khandwa Road, Indore-452001

1To whom all correspondence should be addressed.

Abstract

The supply of 0.01 and 1.0 mM mercuric chloride inhibited substantially in vivo as well as in vitro the total nitrate reductase activity and endogenous nitrate pool in excised bean leaf segments. The in vitro specific activity remained unchanged. Percent inhibition in nitrate reductase activity by metal increased with incubation period. The toxic effect of mercury was overcome completely by the supply of glutathione, cysteine and sucrose and partially by molybdic acid and NADH. It is suggested that Hg has an inhibitory role on MR activity in bean leaf segements.